GIFT: Genomic integrated force for type II diabetes

• Unité de recherche: PCRD EU
• Numéro de projet: 99.0698
• Titre du projet anglais: GIFT: Genomic integrated force for type II diabetes

Mots-clé (Anglais)

Diabetes; genetics; glucose metabolism; insulin

Autre Numéro de projet (Anglais)

EU project number: QLG2CT199900546

Programme de recherche (Anglais)

EU-programme: 5. Frame Research Programme - 1.1.8 Generic R&D activities

Description succincte (Anglais)

See abstract

Autres indications (Anglais)

Full name of research-institution/enterprise:
Université de Lausanne
Institut de Pharmacologie et de Toxicologie

Partenaires et organisations internationales (Anglais)

Coordinator: CNRS, Lille (F)

Résumé des résultats (Abstract) (Anglais)

I have carried out the following studies, which in aggregate address all subtasks and deliverables of WP5:

1. Established a fully annotated list of ~1000 genes involved in metabolic, signalling and differentiation pathways controlling glucose homeostasis.
2. Tested oligo (50-mers) microarrays and different labelling techniques for cRNA preparations and hybridization. Chose not to further use long oligo arrays.
3. Generated ~630 cDNAs from the gene list mentioned in 1. by RT-PCR, subcloning and sequence verification. These cDNAs have been amplified and have been printed as microarrays on glass slides.
4. Generated a cDNA library from fat obtained from control, obese and diabetic mice. Sequenced ~1000 individual clones representing 550 different genes, of which 77 are not represented in publicly available EST data bases. These cDNA will complete the list of genes to be printed on microarrays.
5. Generated a cDNA library from subcutaneous and visceral fat obtained from control, obese and diabetic human patients.
6. Completed the metabolic characterization of genetically identical mice under high fat diet and which develop different phenotypes: obesity with diabetes, no obesity but diabetes and no obesity nor diabetes. Stored liver, muscle, adipose tissue, hypothalamus from large number of these mice for microarray analysis.

Références bases de données (Anglais)

Swiss Database: Euro-DB of the
State Secretariat for Education and Research
Hallwylstrasse 4
CH-3003 Berne, Switzerland
Tel. [phone]
Swiss Project-Number: 99.0698