CHRONO: Chub and herring as reference materials for ortho and non-ortho chlorobiphenyls

• Forschungsstelle: EU FRP
• Projektnummer: 99.0153-2
• Projekttitel Englisch: CHRONO: Chub and herring as reference materials for ortho and non-ortho chlorobiphenyls

Schlüsselwörter (Englisch)

Reference materials; PCB; ortho chorobiphenyls; non-ortho chorobiphenyls; multidimentional gas-chromatography; herring; chub

Alternative Projektnummern (Englisch)

EU project number: SMT4-CT98-2243

Forschungsprogramme (Englisch)

EU-programme: 4. Frame Research Programme - 2.2 Measurements and testing

Kurzbeschreibung (Englisch)

See abstract

Weitere Hinweise und Angaben (Englisch)

Full name of research-institution/enterprise:
EPF Lausanne
Institut de Génie de l'Environnement
Génie Sanitaire - Ecotoxicologie

Partner und Internationale Organisationen (Englisch)

Coordinator: RIVO DLO (NL)

Abstract (Englisch)

The Laboratory of Ecosystem Management (GECOS) from the EPFL participated with success in the certification of the content of 12 ortho and 4 non-ortho chlorobiphenyls (CBs) in two different species of fish.
1) Twelve ortho-CBs (IUPAC nos. 28, 52, 101, 105, 118, 128, 138, 149, 153, 156, 170 and 180) were certified in a herring reference material.
After saponification of the sample and extraction with hexane, the extracts were cleaned with sulphuric acid and on column chromatography with silica-gel. The identification was done with a multidimensional GC (2 different columns) and ECD detection.
2) Four non-ortho CBs (IUPAC nos. 77, 81, 126 and 169) were certified in a chub reference material.
The CBs were extracted from the fish matrix by a mixture of acetone - hexane with an Ultraturrax in an ultrasonic bath. Extracts were cleaned with sulphuric acid and on column chromatography with acidic silica-gel. The non-ortho CBs were separated from the other CBs by carbon column chromatography. The identification was done with a multidimensional GC (2 different columns) and ECD detection. The amounts certified of the non-ortho CBs were very low, between 1.6 and 184.2 ng.kg-1.

Datenbankreferenzen (Englisch)

Swiss Database: Euro-DB of the
State Secretariat for Education and Research
Hallwylstrasse 4
CH-3003 Berne, Switzerland
Tel. [phone]
Swiss Project-Number: 99.0153-2