Analysis of molecular factors affecting variability in BSE and Scrapie susceptibility

• Research unit: EU RFP
• Project number: 97.0579-1

Key words (English)

BSE; PRNP; cattle; association study; polymorphism; genetic mapping; microsatellites

Alternative project number (English)

EU project number: FAIR5-CT97-3311

Research programs (English)

EU-programme: 4. Frame Research Programme - 4.3 Biomedical/Health research

Short description (English)

See abstract

Partners and International Organizations (English)

Coordinator: Roslin Institute Edinburgh(UK)

Abstract (English)

BASED ON THE HYPOTHESIS THAT FACTORS OTHER THAN EXPOSURE TO THE INFECTIOUS AGENT ALONE ARE RESPONSIBLE FOR THE DISEASE, THE PURPOSE OF THIS STUDY WAS TO FIND GENETIC ASSOCIATIONS BETWEEN BSE AND MARKERS OF THE PRNP AND/OR BOLA REGION. THE ANALYSIS FOCUSED ON AFFECTED CASES AND HEALTHY CONTROLS. HIGHLY POLYMORPHIC MICROSATELLITES SURROUNDING THE TWO REGIONS OF INTEREST WERE USED AS GENETIC MARKERS. THESE MARKERS WERE SELECTED BASED ON PREVOUSLY CONDUCTED MARKER MAPPING ANALYSES.
SCHEDULED GOALS FOR THE PERIOD
ACCORDING TO THE PROJECT PROPOSAL, THE GOALS FOR THE REPORT PERIOD WERE AS FOLLOWS:
SUBMIT MANUSCRIPT ON HIGHER RESOLUTION RADIATION HYBRID MAPPING OF BTA13.
CONTINUE SEQUENCE COMPARISON OF PRNP 5' REGULATORY REGION IN SELECTED BSE CASES AND CONTROLS.
CONCLUDE RESEARCH PROJECT.
RESULTS
THIS FINAL REPORT COVERS THE MAIN RESULTS OF THE RESEARCH PROJECT. THE REPORT IS DEVIDED INTO SEVEN SECTIONS:
1: GENETIC MAPPING OF PRNP
2: ASSIGNMENTS OF SIX TYPE I MARKERS TO BTA13
3: RADIATION HYBRID MAPPING OF BTA13
4: VARIATION IN PRNP CODING REGION
5: BSE CASE/CONTROL STUDY ON BTA13 AND BTA23
6: SEQUENCE COMPARISON OF THE PRNP 5' REGULATORY REGION
7: OTHER ACTIVITIES

References in databases (English)

Swiss Database: Euro-DB of the
State Secretariat for Education and Research
Hallwylstrasse 4
CH-3003 Berne, Switzerland
Tel. [phone]
Swiss Project-Number: 97.0579-1