Biochemical and genetic analysis of the class II gene transcription machinery and associated factors

• Research unit: EU RFP
• Project number: 96.0072

Key words (English)

Ccr4-Not complex; transcription; yeast; NC2

Alternative project number (English)

EU project number: FMRXCT960064

Research programs (English)

EU-programme: 4. Frame Research Programme - 10.1 Stimulation of training and mobility

Short description (English)

See abstract

Partners and International Organizations (English)

LMU (D), KUN (NL), RUU (NL), IMBB (EL), IMBB (EL), CEA (F)

Abstract (English)

The S.cerevisiae Ccr4-Not complex is a global regulator of transcription that affect gene expression positively and negatively. The NOT genes in particular appear to preferentially repress transcription from promoters which lack a canonical TATA sequence. Not1p is the only protein of the complex that is essential for yeast viability, and consists of two domains that can function when expressed in trans and are associated with different proteins of the Ccr4-Not complex. Because the Ccr4-Not complex has different effects on different core promoters, it is thought to regulate the funcion of TFIID, and several ineractions between the Ccr4-Not complex and components of TFIID have been described.
We pursued this analysis and have obtaine evidence that the C-terminal region of Not1p interacts with the N-terminal region of yTafII130p, a subunit of TFIID, to regulate the association of TBP at promoters.
In an other study aimed at understanding the physiological role of the Ccr4-Not complex, we were able to demonstrate that iticontributes to the regulation of the Msn2-dependent transcription by the RAS/cAMP pathway.
Finally, we were able to show that the putative RNA helicase Dhh1p that contributes to mRNA decapping, is associated with the N-terminal region of Not1p, as are Caf1p and Ccr4p, that are involved in mRNA deadenylation, most likely within the context of Ccr4-Not complexes. These results suggest that the Ccr4-Not complex might regulate RNA degradation in addition to transcription.

References in databases (English)

Swiss Database: Euro-DB of the
State Secretariat for Education and Research
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CH-3003 Berne, Switzerland
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Swiss Project-Number: 96.0072