Entwicklung einer optimalen Strategie für das Monitoring antibiotikaresistenter Keime von Nutztieren in der Schweiz

• Forschungsstelle: BLV
• Projektnummer: 1.02.02
• Projekttitel Englisch: Development of an optimal strategy for monitoring animicrobial resistance in bacteria from food animals in Switzerland

Schlüsselwörter (Deutsch)

Antimikrobielle Resistenz, Monitoring, Campylobacter

Schlüsselwörter (Englisch)

Antimikrobielle Resistenz, Monitoring, Campylobacter

Kurzbeschreibung (Englisch)

The objective of this project is the development of the scientific, conceptual and practical basis for the implementation of routine antibiotic resistance monitoring in food producing animals in Switzerland. Optimal sampling designs will be developed based on statistical, microbiological and economical analyses. As a model agent, Campylobacter jejuni and C. coli and their respective resistance patterns will be used. These bacteria were selected due to their relatively simple epidemiology along the pro-duction chain avoiding complex dynamics in the early phase of the project. The methodological as-pects to be investigated are the distribution of resistance variability between bacteriological colonies, animals and farms as well as the correlation between resistance levels of various sampling points along the stable-to-table chain. The latter is investigated by defining a grid of sampling points. The grid consists of three sampling points each for poultry, cattle and pigs, and one point for imported meat, so that in total, twelve grid points are defined. The types of samples collected are: Faecal samples (collected at slaughter or on farm); carcass samples collected at slaughter; meat samples col-lected at retail level. The samples originate from existing monitoring programmes, from special sam-pling campaigns or from other ongoing research projects. The results of all grid points are used for the development of a model that can be used for selecting optimal sampling points. Each sampling point is described by a set of characteristics, including likelihood of resistance detection and cost. This model will then be expanded to accommodate other agents than Campylobacter. The results from the project will form the basis for the development of a resistance monitoring programme in food animals in Switzerland. For the implementation, close collaboration is envisaged with veterinary and public health authorities.
The results of the project will further be used to conduct an exposure assessment for consumers in Switzerland with respect to resistant Campylobacter strains from meat.

Projektziele (Englisch)

The objective of this project is the development of the scientific, conceptual and practical basis for the implementation of routine antibiotic resistance monitoring in food producing animals in Switzerland. Optimal sampling designs will be developed based on statistical, microbiological and economical analyses. As a model agent, Campylobacter jejuni and C. coli and their respective resistance patterns will be used. These bacteria were selected due to their relatively simple epidemiology along the pro-duction chain avoiding complex dynamics in the early phase of the project. The methodological as-pects to be investigated are the distribution of resistance variability between bacteriological colonies, animals and farms as well as the correlation between resistance levels of various sampling points along the stable-to-table chain. The latter is investigated by defining a grid of sampling points. The grid consists of three sampling points each for poultry, cattle and pigs, and one point for imported meat, so that in total, twelve grid points are defined. The types of samples collected are: Faecal samples (collected at slaughter or on farm); carcass samples collected at slaughter; meat samples col-lected at retail level. The samples originate from existing monitoring programmes, from special sam-pling campaigns or from other ongoing research projects. The results of all grid points are used for the development of a model that can be used for selecting optimal sampling points. Each sampling point is described by a set of characteristics, including likelihood of resistance detection and cost. This model will then be expanded to accommodate other agents than Campylobacter. The results from the project will form the basis for the development of a resistance monitoring programme in food animals in Switzerland. For the implementation, close collaboration is envisaged with veterinary and public health authorities.
The results of the project will further be used to conduct an exposure assessment for consumers in Switzerland with respect to resistant Campylobacter strains from meat.

Abstract (Deutsch)

The objective of this project is the development of the scientific, conceptual and practical basis for the implementation of routine antibiotic resistance monitoring in food producing animals in Switzerland. Optimal sampling designs will be developed based on statistical, microbiological and economical analyses. As a model agent, Campylobacter jejuni and C. coli and their respective resistance patterns will be used. These bacteria were selected due to their relatively simple epidemiology along the pro-duction chain avoiding complex dynamics in the early phase of the project. The methodological as-pects to be investigated are the distribution of resistance variability between bacteriological colonies, animals and farms as well as the correlation between resistance levels of various sampling points along the stable-to-table chain. The latter is investigated by defining a grid of sampling points. The grid consists of three sampling points each for poultry, cattle and pigs, and one point for imported meat, so that in total, twelve grid points are defined. The types of samples collected are: Faecal samples (collected at slaughter or on farm); carcass samples collected at slaughter; meat samples col-lected at retail level. The samples originate from existing monitoring programmes, from special sam-pling campaigns or from other ongoing research projects. The results of all grid points are used for the development of a model that can be used for selecting optimal sampling points. Each sampling point is described by a set of characteristics, including likelihood of resistance detection and cost. This model will then be expanded to accommodate other agents than Campylobacter. The results from the project will form the basis for the development of a resistance monitoring programme in food animals in Switzerland. For the implementation, close collaboration is envisaged with veterinary and public health authorities.
The results of the project will further be used to conduct an exposure assessment for consumers in Switzerland with respect to resistant Campylobacter strains from meat.

Publikationen / Ergebnisse (Deutsch)

Stärk K.D.C., Regula G. (2003): Antibiotikaresistenz im Veterinärbereich: Entwicklung in der Schweiz seit 1998. Suisseporcs-Informationen 6/2003; 18-19

Ledergerber, U. (2003): Antibiotika-Resistenzen bei Mastschweinen. Swissporcs Informationen 5/2003Regula, G., Stärk, K.D.C. (2003): Überwachung der Antibiotikaresistenz bei Nutztieren. Tagungsband GST Tagung, Oktober 2003.

Regula, R., Lo Fo Wong, D.M.A., Ledergerber, U., Stephan, R., Danuser, J., Bissig-Choisat, B., Stärk, K.D.C. (?): Opitmization of an antimicrobial resistance monitoring program for campylobacter in poultry by Monte Carlo simulation. Preventive Veterinary Medicine, eingereicht.

Publikationen / Ergebnisse (Englisch)

Ledergerber U., Regula G., Stephan R., Danuser J., Bissig B., Stärk K.D.C (2003): Risk factors for antimicrobial resistance in poultry meat. BMC Public Health 3:39, www.biomedcentral.com

Ledergerber, R., Regula, G., Danuser, J., Bissig, B., Stephan, R., Stärk, K.D.C. (2003): Zoonotic pathogens and antimicrobial resistance in "animal-friendly" pig production systems in Switzerland. Proceedings 5th Int. Symposium on Epidemiology and Control of foodborne pathogens in pork, 1.-4. Oktober 2003, Kreta, Griechenland.

Regula, G., Lo Fo Wong, D.M.A., Stephan, R., Bissig, B., Danuser, J., Ledergerber, U., Stärk, K.D.C. (2003): Optimization of an antimicrobial resistance monitoring program, using Campylobacter spp. in poultry production as an example. Proceedings, 10th ISVEE, 17.-21. November 2003, Vina del Mar, Chile

Regula, G., Ledergerber, U., Stephan, R., Schuppers, M.E., Bissig, B., Danuser, J., Stärk, K.D.C. (2003): Risk factors for antibiotic resistance in Campylobacter spp. from finishing pigs. Proceedings, 10th ISVEE, 17.-21. November 2003, Vina del Mar, Chile

Regula, G., Stephan, R., Danuser, J., Bissig, B., Ledergerber, U., Lo Fo Wong, D., Stärk, K.D.C. (2003): Reduced antibiotic resistance to fluoroquinolones and streptomycin in "animal-friendly" pig fattening farms in Switzerland. The Veterinary Record, January 18, 2003

Ledergerber, U., Regula, G., Stephan, R., Danuser, J., Bissig, B., Stärk, K.D.C. (2003): Risk factors for antibiotic resistance in Campylobacter spp. isolated from raw poultry meat in Switzerland. BMC Public Health, 3:39

Regula G., Ledergerber U., Stephan R., Danuser J., Lo Fo Wong D., Bissig-Choisat B. and Stärk K.D.C. (2004): Antibiotic resistance in Campylobacter spp. in Swiss poultry production. Proceedings of the Society of Veterinary Epidemiology and Preventive Medicine, March 24-26, 2004, Martigny, Switzerland.