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Unité de recherche
METAS
Numéro de projet
F-5235.30154
Titre du projet
Kalibrierverfahren Bioaerosolmonitore

Textes relatifs à ce projet

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Mots-clé
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Description succincte
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Objectifs du projet
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CatégorieTexte
Mots-clé
(Anglais)
pollen monitoring, calibration, counting efficiency, reference aerosols, pollen taxa identification
Description succincte
(Anglais)

More than 100 million Europeans suffer from allergic rhinitis and 70 million from asthma, with the associated annual costs estimated at EUR 50-150 billion. Accurate pollen monitoring and forecasting is needed to prevent, diagnose and treat pollen allergy and to underpin the direction of R&D for new therapies. To support this, some of the leading European Meteorological Services launched the "EUMETNET Auto Pollen Programme", a self-funded collaboration between 11 EU countries, third parties from 9 additional European countries and two international groups. The goal of the EUMETNET programme is to establish an automatic pollen monitoring network in Europe for the real-time measurement of the number concentration of airborne pollen particles and the identification of their taxa. However, currently, neither calibration nor any quality assurance procedures exist to ensure measurement accuracy and reproducibility for automatic pollen monitors.

Currently, the counting of pollen particles and identification of their taxa relies on manual methods, i.e. collection of pollen on suitable substrates and subsequent inspection with a microscope. These methods provide poor time resolution, typically on a weekly basis, are time consuming and entail high personnel costs. In contrast, new automatic pollen monitoring instruments provide real-time measurements, are suited for unattended operation and will help to provide denser spatial coverage with lower running costs. However, the measurement accuracy and reproducibility of these instruments is not well known, since no reference aerosols or calibration methods currently exist for automatic pollen monitoring instruments.

Objectifs du projet
(Anglais)

This project will go beyond the state of the art by i) generating single and multi-component pollen reference aerosols under well-defined environmental conditions (i.e. temperature and humidity) to simulate the seasonal variability in the morphology of pollen particles; ii) generating internal mixtures of pollen with non-biological particles found in ambient air (e.g. dust particles); iii) developing traceable calibration procedures for determining the counting efficiency of automatic pollen monitors based on model polystyrene latex (PSL) particles and real pollen particles.

The reference aerosols from i) and ii) will be used to train the artificial intelligence algorithms used for particle identification while the reference aerosols from iii) for quantifying the counting efficiency of the monitors. The project’s development of calibration procedures for automatic pollen monitors will improve data comparability in Europe. This will result in accurate, real-time data becoming readily available while at the same time reducing operational costs (compared to the current time-consuming, manual methods for monitoring pollen). Moreover, the methods developed for pollen monitors could be used in future applications for the detection of other bioaerosols, such as bacterial and fungal spores, many of which are human and plant pathogens.