Skin Bioprinting: an innovative approach to produce standardized skin models on demand

• Unité de recherche: INNOSUISSE
• Numéro de projet: 14331.1;9 PFLS-LS
• Titre du projet anglais: Skin Bioprinting: an innovative approach to produce standardized skin models on demand

Description succincte (Allemand)

Skin Bioprinting: an innovative approach to produce standardized skin models on demand

Description succincte (Anglais)

Skin Bioprinting: an innovative approach to produce standardized skin models on demand

Résumé des résultats (Abstract) (Allemand)

3D cell cultivation is gaining momentum not only in academic research but also in industry. While the translation from 2D to 3D cell culture in pharma industry is still in its infancy the cosmetic industry is forced to employ in vitro organotypical skin models for substance evaluation due to an animal test ban starting from 2013. Therefore, there is a huge demand in Switzerland and Europe for reliable standardized skin models that are produced cost-effective. Commercially available skin models are very expensive and rather simple in their composition (limited biological relevance). Furthermore, the need for customized skin models to address specific questions or disease models is increasing (concrete inquiry of Mibelle and DSM). The substance of the project is to implement a bioprinting device for the automated production of skin models, which provides not only the instrument but also SOPs with proven biological relevance and a disposable accompanying biomaterial kit. The technology applied allows the spatial control in a layer by layer deposition process of scaffold material, cells and bioactives, providing so an organomimetic approach. The deliverables of the project is an all-in-one product for full-thickness skin model production including 1) skinprinter device providing a sterile working environment and an OCT-system for in situ quality control, 2) cell-compatible, printable universal BioInk as disposable ready to use kit, 3) standard operating procedure (SOP) and application note.

Résumé des résultats (Abstract) (Anglais)

3D cell cultivation is gaining momentum not only in academic research but also in industry. While the translation from 2D to 3D cell culture in pharma industry is still in its infancy the cosmetic industry is forced to employ in vitro organotypical skin models for substance evaluation due to an animal test ban starting from 2013. Therefore, there is a huge demand in Switzerland and Europe for reliable standardized skin models that are produced cost-effective. Commercially available skin models are very expensive and rather simple in their composition (limited biological relevance). Furthermore, the need for customized skin models to address specific questions or disease models is increasing (concrete inquiry of Mibelle and DSM). The substance of the project is to implement a bioprinting device for the automated production of skin models, which provides not only the instrument but also SOPs with proven biological relevance and a disposable accompanying biomaterial kit. The technology applied allows the spatial control in a layer by layer deposition process of scaffold material, cells and bioactives, providing so an organomimetic approach. The deliverables of the project is an all-in-one product for full-thickness skin model production including 1) skinprinter device providing a sterile working environment and an OCT-system for in situ quality control, 2) cell-compatible, printable universal BioInk as disposable ready to use kit, 3) standard operating procedure (SOP) and application note.