Partners and International Organizations
(English)
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AT, BG, CH, CY, CZ, DE, ES, FR, GR, HU, IL, IT, LU, PL, PT, RS, SE, SI
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Abstract
(English)
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Neofusicoccum parvum, anamorph of Botryosphaeria parva (Botryosphaeriaceae, Ascomycetes) is a plant pathogenic fungus, saprophyte and endophyte, with a worldwide distribution on a wide range of woody hosts. Recently, an increasing number of species of Botryosphaeriaceae have been associated with grapevine decline worldwide (Lehoczky, 1974; Larignon and Dubos 2001, Phillips 2002, Urbez-Torres et al. 2005 ). Thirteen strains of N. parvum isolated from vine showing decline symptoms, esca and or BDA symptoms in Portugal and France were screened for phytotoxic activity. One strain was choosen for a high-scale fermentation culture for chemical studies. N. parvum, Bourgogne 2-116 strain, isolated from a nursery plant was grown on a hundred PDA Pétri for seven days at 28°C. Chemical separation and purification of the crude extract led to the isolation and identification of 12 compounds, among them (-)-mellein, cis and trans hydroxymellein and 3 hydroxymellin. The first three compounds have been reported from B. obtusa, B.ribis and B.mamane (Djoukeng et al. 2007). A new family of compounds related to terremutic acid, is described for the first time from Botryospaeriaceae, terremutin, terremutine hydrate, succinic acid-terremutine hydrate, although most of these compounds have been isolated from Aspergillus sp. During this study, the investigation of medium on metabolites biosynthesis and the capacity of N. parvum to biosynthesise the same compounds on grapevine wood was reproduced and medium and conditions was performed in order to optimise conditions for toxins production. References Lehoczky J., 1974. Acta Phytopathologica Academiae Scientiarum Hungaricae 9, 319-327. Larignon P. and B. Dubos, 2001. Wines & Vines 82, 86-89. Phillips A.J.L., 2002. Phytopathologia Mediterranea 41, 3-18. J. D Djoukeng, S.Polli, P. Larignon and E. Abou-Mansour, European Journal of Plant Pathology Volume 124, Number 2, 303-308, DOI: 10.1007/s10658-008-9419-6 Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine Urbez J.R., G.M. Leavitt and W.D. Gubler, 2005. Phytopathologia Mediterranea 44, 87
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