NMR structure determination of dsRBDs in complex with siRNAs and miRNA.

Fonte Standard Gras
Identifiant

Unité de recherche

INNOSUISSE

Numéro de projet

11329.1;10 PFLS-LS

Titre du projet

NMR structure determination of dsRBDs in complex with siRNAs and miRNA.

Titre du projet anglais

NMR structure determination of dsRBDs in complex with siRNAs and miRNA.

Textes relatifs à ce projet

	Allemand	Français	Italien	Anglais
Description succincte	-	-	-
Résumé des résultats (Abstract)	-	-	-

Textes saisis

Catégorie	Texte
Description succincte (Anglais)	NMR structure determination of dsRBDs in complex with siRNAs and miRNA.
Résumé des résultats (Abstract) (Anglais)	The discovery of RNA silencing has opened up a completely new avenue for therapy. The main goal of this project is to determine the structure of the dsRBDs of three proteins which are part of RISC (RNA Induced Silencing Complex), namely Dicer, TRBP (TAR RNA Binding protein) and PACT (Protein Activator of PKR), in their free state and in complex with high affinity siRNA and pre-miRNA. The structure of these dsRBD-RNA complexes will be used to design new siRNA- and miRNA-based therapeutics, which will eventually be tested in in vivo mouse models

Catégorie

Texte

Description succincte
(Anglais)

NMR structure determination of dsRBDs in complex with siRNAs and miRNA.

Résumé des résultats (Abstract)
(Anglais)

The discovery of RNA silencing has opened up a completely new avenue for therapy. The main goal of this project is to determine the structure of the dsRBDs of three proteins which are part of RISC (RNA Induced Silencing Complex), namely Dicer, TRBP (TAR RNA Binding protein) and PACT (Protein Activator of PKR), in their free state and in complex with high affinity siRNA and pre-miRNA. The structure of these dsRBD-RNA complexes will be used to design new siRNA- and miRNA-based therapeutics, which will eventually be tested in in vivo mouse models