Partners and International Organizations
(English)
|
A, B, CZ, DK, FIN; F, D, H, IRL, I, LV, NL, N, P, SI, E, S, CH, GB
|
Abstract
(English)
|
The aim of our COST-D16 project has been to prepare enzyme fingerprinting chips for enzyme characterization, quality control and medical diagnostics applications. The project involved two organic synthesis teams who had previously collaborated on similar projects (Reymond - University of Berne, Switzerland; Crotti - University of Pisa, Italy), and two microbiology teams, one acadamic (Duchiron - CNRS Rennes, Frances) and one industrial (Lefèvre - Protéus SA, Nîmes, France). The first project phase had demonstrated the feasibility of enzyme fingerprinting using soluble substrates in microtiter-plates.1 In the final project phase we have proceeded to apply enzyme fingerprinting on solid support, and developed two independent methods: enzyme substrates adsorbed on silicagel surfaces,2 and microarray layout on glas slides.3 We have also reported a new method for multi-enzyme fingerprinting of extromophiles using substrate cocktails,4 which provides a rapid functional classification method based on enzymatic activity of the microbial strains.5 Support of COST and OFES has thus lead to a highly fruitful project. Latest publications which have resulted from this COST-D16 working group: a) J. Grognux, J.-L. Reymond, ChemBioChem 2004, 5, 826-831; b) D. Wahler, O. Boujard, F. Lefèvre, J.-L. Reymond, Tetrahedron 2004, 60, 703-710; c) J. Grognux, D. Wahler, E. Nyfeler, J.-L. Reymond, Tetrahedron Asym. 2004, 15, 2981-2989. 2 P. Babiak, J.-L. Reymond, Anal. Chem. 2005, 77, 373-377 (with cover picture). 3 J. Grognux, J.-L. Reymond, Poster presentation ACS meeting, San Diego, 13-17 March 2005. 4 a) J.-P. Goddard, J.-L. Reymond, J. Am. Chem. Soc. 2004, 126, 11116-11117; b) Y. Yongzheng, J.-L. Reymond, MolBiosys 2005, submitted. 5 R. Sicart, J.-P. Goddard, M. Mazel, C. Audiffrin, L. Fourage, G. Ravot, D. Wahler, F. Lefèvre, J.-L. Reymond, submitted.
|
