DYNAMIC-TRANS-COMPS: Transcriptional regulatory complexes: dynamic functional interplay in vivo and structural organisation

• Unité de recherche: PCRD EU
• Numéro de projet: 02.0017
• Titre du projet anglais: DYNAMIC-TRANS-COMPS: Transcriptional regulatory complexes: dynamic functional interplay in vivo and structural organisation

Mots-clé (Anglais)

Education; Training; Scientific Research; Social Aspects

Autre Numéro de projet (Anglais)

EU project number: HPRN-CT-2002-00261

Programme de recherche (Anglais)

EU-programme: 5. Frame Research Programme - 4.1.1 Research training networks

Description succincte (Anglais)

See abstract

Résumé des résultats (Abstract) (Anglais)

The objective of this proposal is to understand the function and structure of the transcription regulatory complexes (TFIID, B-TFIID, CCR4-Not, NC2, PC4, and PC6), which modulate both positively and negatively RNA polymerase II transcription. The respective roles of each complex in programming gene expression patterns during growth and development will be adressed in vivo and the molecular mechansims underlying their activity will be studied by functional in vitro assays. Focus will be placed on the dynamic interplay between complexes, the identification of target genes their structural organisation, and how post-translational modifications modulate stability and activity of their constituent subunits. Our collaborative approach will exploit yeast and mammalian genetic models along with biochemical and biophysical studies.

Specific aims include:
1. Analysis of genetic interactions between the above factors in vivo in yeast and mutant mice/mammalian cell lines. Physical interactions in vitro through biochemical studies with the respective complexes and/or their subunits;
2. Characterisation of the respective roles of each complex in vivo and identification of target genes by chromatin immunoprecipitation and analysis of knock out lines/strains;
3. Analysis of interplay between factors during growth and differentiationvia yeast/mammalian genetic models and in vitro;
4. Investigation of transcription factor regulation through changes in subunit stability induced by sumoylation/ubiquitination;
5. Electron microscopy of the B-TFIID and CCR4-Not complexes. Understanding the organisation, function, and interplay of transcription factor complexes is a major challenge in modern biology. The strength of our network to tackle these questions lies in the multidisciplinary expertise of the constituent groups and their ability to address different aspects of the problem. This integrated aproach will deliver important new contributions.

Références bases de données (Anglais)

Swiss Database: Euro-DB of the
State Secretariat for Education and Research
Hallwylstrasse 4
CH-3003 Berne, Switzerland
Tel. [phone]
Swiss Project-Number: 02.0017