PREGENE: Precision engineering of plant genes

• Forschungsstelle: EU FRP
• Projektnummer: 00.0187-2
• Projekttitel Englisch: PREGENE: Precision engineering of plant genes

Schlüsselwörter (Englisch)

Arabidopsis; butafenacil; Biotechnology; Environmental Protection; Innovation; Technology Transfer; Life Sciences; Medicine; Health; Waste Management

Alternative Projektnummern (Englisch)

EU project number: QLRT-2000-00365

Forschungsprogramme (Englisch)

EU-programme: 5. Frame Research Programme - 1.1.3 The 'cell factory'

Kurzbeschreibung (Englisch)

See abstract

Weitere Hinweise und Angaben (Englisch)

Full name of research-institution/enterprise:
Friedrich Miescher Institut
Zweigniederlassung der Novartis Forschungsstiftung

Partner und Internationale Organisationen (Englisch)

Coordinator: Max-Planck-Institut für Züchtungsforschung, Köln (D)

Abstract (Englisch)

The quality of transgene expression is influenced by the position of integration in the genome and the complexity of the integrated DNA. Today' s methods cannot control both parameters, but gene targeting would be the solution. However, this technology is not available in higher plants. Gene targeting relies on homologous recombination. Progress in the general understanding of homologous recombination and the discovery of highly efficient gene targeting in a moss allow new approaches to improve gene targeting in plants. Key parameters that differ in efficient and inefficient systems are analysed, appropriate genes and/or features transferred to low efficiency gene-targeting systems and the function is confirmed in the modified systems. Concepts shown to improve gene targeting are transferred to commercially important systems as a first step towards application.

Datenbankreferenzen (Englisch)

Swiss Database: Euro-DB of the
State Secretariat for Education and Research
Hallwylstrasse 4
CH-3003 Berne, Switzerland
Tel. [phone]
Swiss Project-Number: 00.0187-2