Partners and International Organizations
(English)
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AT, BE, BG, CY, CZ, FI, FR, DE, EL, HU, IE, IL, IT, LU, NL, NO, PL, PT, RO, CS, SK, ES, SE, CH, UK
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Abstract
(English)
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In previous years of this COST 843 action, RAPD (random amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism) were used for distinguishing different Prunus species and mutants derived there from. From these two methods AFLP was the only one to discriminate clearly cultivars and mutants. For the distinction of cultivars, mi-crosatellites or SSR (single sequence repeats) have been successfully applied for a wide range of plant species. In programmes conserving old gene resources such methods are essential for the identification of individuals. Therefore, during the final project period (year 2005), sixty-one strawberry cultivars and selections (Fragaria ssp.) were analysed using 8 microsatellites and the multiplex fluorescence PCR method (J.E. Frey et al. 2004: Plant Breeding 123: 63-65). The analysis of fragments revealed clear distinction of cultivars as well as the identification of duplicates or mislabelled plants. By this, two different sources of 'Surprise des Halles' were shown to be two different genotypes (not identical cultivars), whereas the cultivars 'Amazone' and 'Jucunda', which are described in the literature as synonymes (http://www.agr.unian.it/ricerca/prog_ric/Wg1/ GeneticResources/ Euro-pean%20Germplasm.htm) were clearly distinguished as different genotypes. DNA extractions from in vitro cultures and from mother plants of the cultivar 'Darselect' gave identical DNA fragments, confirming the reliability of this method.For the identification and distinction of strawberry cultivars and species (e.g. F. vesca and F. moschata) multiplex microsatellite markers proofed to be a potential method.
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