EC ENZYMES: Industrial biocatalysis with new oxygenases in a novel electro-enzyme reactor

• Unité de recherche: PCRD EU
• Numéro de projet: 00.0399
• Titre du projet anglais: EC ENZYMES: Industrial biocatalysis with new oxygenases in a novel electro-enzyme reactor

Mots-clé (Anglais)

Monooxygenase; electrochemistry; bioinformatics; directed evolution;
Biotechnology; Environmental Protection; Innovation; Technology Transfer; Life Sciences; Medicine; Health; Waste Management

Autre Numéro de projet (Anglais)

EU project number: QLK3-2000-00725

Programme de recherche (Anglais)

EU-programme: 5. Frame Research Programme - 1.1.3 The 'cell factory'

Description succincte (Anglais)

See abstract

Partenaires et organisations internationales (Anglais)

Coordinator: BASF Aktiengesellschaft (D)

Résumé des résultats (Abstract) (Anglais)

The objective of this proposal is to obtain novel functional biocatalysts for regio- and enantio-selective oxidation working in a 'reactor plugged to a wall socket'. A central part is the isolation of the responsible part of monooxygenases and the combination with an electrochemical regeneration system. Our aim is to use sophisticated mediator technology to develop biocatalysts which, in an industrial setting, are competitive to chemical catalysis used in hydroxylation.
Together with an integration of mechanistic information of the enzymes and genetic engineering techniques, a large number of mutants prepared by directed evolution will be analysed by novel massive parallel screening methods. Based on the developments a basically new electro-enzymatic reactor will be constructed for chemical bulk synthesis of oxygenated fine chemicals.

Références bases de données (Anglais)

Swiss Database: Euro-DB of the
State Secretariat for Education and Research
Hallwylstrasse 4
CH-3003 Berne, Switzerland
Tel. [phone]
Swiss Project-Number: 00.0399