Schlüsselwörter
(Deutsch)
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Aeromonas spp., Plesiomonas spp., Lebensmittel, PCR
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Schlüsselwörter
(Englisch)
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Aeromonas spp., Plesiomonas spp., food, PCR
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Kurzbeschreibung
(Englisch)
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Thirty years ago, Listeria monocytogenes, Campylobacter jejuni, Aeromonas hydrophila, Plesiomonas shigelloides, Vibiro parahaemolyticus and Yersinia enterocolitica were not known; now these are well-established foodborne pathogens that we must control.
Mesophilic Aeromonas spp. (including Aeromonas hydrophila) are Gram negative bacteria which are recognized nowadays as a group of emerging pathogens. They have been isolated from environmental, food, animals, fish and human clinical materials. They were also implicated in human infections around the world especially in diarrheic children and immunodeppressive populations.
Methods for the detection and identifications of pathogenic isolates of these bacteria are lengthy and laborious especially from food samples. Therefore, the need of the development of a rapid and sensitive method for its detection, identification and epidemiological characterisation is urgent for food microbiologist.
A considerable number of PCR-assays have been developed, but they have been applied most often to clinical and environmental samples and more rarely for the detection of foodborne microorganisms.
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Projektziele
(Englisch)
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1- The rapid detection and identification of pathogenic mesophilic Aeromonas spp. by using molecular techniques such as PCR.
2- The enumeration of pathogenic isolates of mesophilic Aeromonas spp. by In situ hybirdization of colonies with a specific probe to a virulence factor gene of the pathogens.
3- The epidemiological characterisation of the pathogenic mesophilich Aeromonas spp. by using molecular typing methods such als Pulsed Field Gel Electrophoresis (PFGE), Random Amplification Polymorphism DNA (RAPD).
4- Introduction of PCR technique in routine detection of foodborne pathogens.
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Abstract
(Englisch)
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The newly developed PCR method indicated that 37 (61%) of the 61 reference strains were positive with the primer cocktail master mixture, and 34 (58%) of 59 environmental isolates, 93 (66%) of 141 food isolates, and 100 (67%) of 150 clinical isolates from around the world carried a virulence factor when primers AHCF1 and AHCR1 were used. In conclusion, this PCR-based method is rapid, sensitive, and specific for the detection of virulence factors of Aeromonas spp. It overcomes the handicap of time-consuming biochemical and other DNA-based methods.
An overall 48% of the tested food samples were contaminated with potential pathogenic Aeromonas spp.
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Publikationen / Ergebnisse
(Englisch)
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Bin Kingombe, Cesar Isigidi, Huys, Geert, Tonolla, Mauro, Albert, M. John, Swings, Jean, Peduzzi, Raffaele, Jemmi, Thomas (1999): PCR Detection, Characterization, and Distribution of Virulence Genes in Aeromonas spp. Applied and Environmental Mictrobiology, Vol. 65, No. 12, 5293-5302.
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